Bauhaus-Universität Weimar

University of Chicago, Division of Biological Sciences, Department of Physiological Chemistry
Koch, Fred Conrad
laboratory instructions. Many classical experiments 
are repeated, such as the determination of isoelectric 
points of proteins, or the effects of pH or salt on enzyme 
activities. In the lectures, the theory underlying these 
methods is discussed and problems are assigned and 
The course on quantitative methods of blood and 
urine analysis is also given with about two lectures a 
week and includes the determination of total nitrogen, 
ammonia, urea, uric acid, calcium, glucose, and acetone 
bodies in urine; and of all of these, in addition to phos¬ 
phate, amino acid nitrogen, alkali reserve, creatine and 
creatinine, and cholesterol in blood. Emphasis is placed 
on accurate work and knowledge of the principles in¬ 
volved in the methods. In the lectures, the chemical 
theory underlying the methods is presented and 
discussed. In the course on quantitative tissue analy¬ 
sis, which is almost exclusively a laboratory course, the 
student performs a complete analysis of some one tissue 
of his own choice for water content, ash, fat, carbo¬ 
hydrate, nitrogen, phosphorus, sulphur, etc., the proce¬ 
dure varying somewhat with the tissue studied. A few 
lectures are given on various procedures used, drying 
and extraction of tissues, precipitations, analytical 
methods, and the calculation of the results. In another 
almost exclusively laboratory course on the quantitative 
analysis of a protein, the student is required to identify 
some common protein by hydrolyzing it and determin¬ 
ing the distribution of the various kinds of nitrogen. 
This involves several determinations of amino and total 
nitrogen, as well as the precipitation and extraction 
procedures on the various fractions. The course on 
biochemical preparations is almost entirely laboratory 
work and involves the preparation of histidine, cystine, 
tyrosin, and leucine from the acid hydrolysis products of 
hemoglobin; the preparation of tryptophane and other 
amino acids from casein after enzymic hydrolysis; and 
the preparation of adrenalin from suprarenal glands. 
The research courses cover the research work done by 
students on particular problems. A joint weekly 
seminar, which all research students are required to 
attend, is conducted by the staff and research students 
in physiology, physiological chemistry, and phar¬ 
The elementary courses are offered during three of the 
four quarters every year; the graduate courses, with the 
exception of that on the biochemistry of internal secre¬ 
tions which is given every other summer, are also offered 
Fig. 3.—Pbivate Research Laboratory showing extractors used in sex hormone work


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