la
amount of chlorine in blood serum, and found they could wort with an accu¬
racy of 0.1 per cent, using 1 c.c. of the solution. I ordered one of
these titration bulbs for use in the Nutrition Laboratory.
I also saw in this laboratory a dog treadmill (see figures 43 and 44)
one of which I secured for our own work with animals.
Ourare experiments.
One of the most important objects of my visit to Budapest was to
obtain more definite information regarding the long series of experiments
with dogs that Tangl and his co-workers have been making on the effect of
curare. The artificial ventilation apparatus of Hans Meyer was used (the
intake of the pump being from outdoors) and the air expired by the cura-
rized dog was carried through an Elster meter to a Zuntz-Geppert gas analy¬
sis apparatus. The dog they were working upon had had the pancreas com¬
pletely extirpated 13 days before and had severe diabetes with sugar in
the urine. No narcotic was used but the animal was tied to the board,
his neck opened, and a Y-shaped tracheal fistula inserted for artificial
respiration. One carotid artery was then connected with a blood pressure
apparatus (a Htfrthle manometer) and the two jugulars used for the injection
of a sugar solution of hormone in one and of curarine in the other. A
cannula was arranged for introducing the sugar solution, the curarine being
injected with a syringe after the dog had been placed in the warm chamber
designed by Tangl. (See figures 45 and 46.) Graphic records of the blocd.
pressure were secured on the kymograph, relative values being thus directly
shown. Tangl uses phosphorus for absorbing the oxygen and says it works
very well indeed. The carbon dioxide is absorbed in 5 minutes, and the
oxygen in 10 or 15 minutes, but he still uses the thermo-barometer of Zuntzi
After the dog is placed in the warm chamber, one half hour is allowed
to elapse before beginning the first experiment. The rectal