Bauhaus-Universität Weimar

Titel:
Handbook for the Physiological Laboratory. Text
Person:
Burdon-Sanderson, John Scott E. Klein Michael Foster T. Lauder Brunton
PURL:
https://digitalesammlungen.uni-weimar.de/viewer/image/lit18583/33/
24 
EPITHELIUM AND ENDOTHELIUM. 
Here and there we may easily perceive that these cells are 
coarsely granular, and that each contains a clear oval nucleus. 
Such coarsely-granular cells increase in number after the pre¬ 
paration has been mounted some time. We may mention that 
the cylindrical cells around the bases of the papillæ are generally 
ciliated. 
Epithelium of Vilfi of Intestine.—In the rabbit we proceed 
as follows :—The animal is killed, the small intestine immediately 
opened, and from the borders (which then curl outwards) we 
remove a small portion with curved scissors as in the previous 
case. This is to be covered with the mucous surface upwards. 
The villi seen exhibit, on their surfaces, a regular mosaic of 
epithelium; at their borders, where the epithelium is in pro¬ 
file, it is seen to consist of regular cylindrical cells. If the 
observation of the mosaic is continued for some time, granu¬ 
lar spherical bodies come into view; at first singly, but after¬ 
wards in numbers, which are raised above the general surface of 
the cells, as may be learnt by using the fine adjustment. These 
spherical bodies have escaped from the cylindrical cells. We 
shall see that it is by this means that the goblet cells already 
mentioned are produced. The epithelial cells on the borders 
of the villi display distinctly the broad, finely-striated border, 
which spreads over their ends like a cuticle. Equally instruc¬ 
tive specimens may be obtained from the intestine of the cat, 
dog, guineapig, rat, or hedgehog. The epithelium of the villi 
may be as successfully studied, while still attached, in a prepara¬ 
tion, mounted in serum, or half per cent, solution of common 
salt. For more prolonged examination, especially if we wish to 
study isolated cells, we put a piece of intestine, cut from the 
rabbit, dog, or cat, into a sherry-yellow solution of bichromate 
of potash, allow it to remain there for one or more days, and 
make our preparation in the manner already described with 
regard to the trachea. In such specimens we find not only 
numerous isolated cells, but also complete villi, and parts of 
the same, on which the epithelium, when its surface is viewed, 
resembles, as in the fresh preparation, a pavement of granular 
cells, each of which contains a relatively large, sharply-bordered, 
and apparently round nucleus. The lines of interstitial substance 
are sharp and dark. At the edges of each villus the epithelial 
cells, are cylindrical, with finely-striated border. Each cell 
consists of granular protoplasm, and contains a sharply-defined 
nucleus, in which a distinct nucleolus is to be seen. 
If we examine attentively the surface of a villus, or of a por¬ 
tion of villus (especially in a preparation from the intestine of 
the dog or cat, which has been allowed to remain in a solution 
of bichromate of potash), we shall find, between the mosaic of
        

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