Bauhaus-Universität Weimar

Handbook for the Physiological Laboratory. Text
Burdon-Sanderson, John Scott E. Klein Michael Foster T. Lauder Brunton
up finely powdered hæmatin in concentrated sulphuric acid. 
A liquid is obtained which is green in thin layers, reddish-brown 
in thicker layers, and gives a brown precipitate when diluted 
with w7ater. This precipitate is easily dissolved in ammonia. 
On evaporating the ammoniacal solution, a bluish-black residue 
with metallic lustre is left, which is free from iron. It may be 
obtained in like manner by acting on methæmoglobin by sul¬ 
phuric acid. The solution of hæmatoin in ammonia exhibits 
four absorption bands. It is admirably shown by the method 
recommended by Professor Stokes, i.e., by extracting with 
ether, blood which has been mixed with acetic acid. The ethe¬ 
real liquid thus obtained exhibits a four-banded spectrum. Of 
these bands, three only are easy to recognize—one in the 
orange, nearer to the red than the reduced hæmatin band ; a 
rather broad band in the green; and a narrow but well-defined 
one in the blue. (See Fig. 195, 3.) 
23. Quantitative Analysis of the Blood, with reference 
to its Corpuscles, Seram, Fibrin, Haemoglobin, Albumin, 
and Salts.—The following summary of the order of proceed¬ 
ing in the analysis of the blood, will be found sufficient for the 
guidance of those who have been previously trained in quanti¬ 
tative methods. The student who has not learnt accuracy by 
practice, in the analysis of bodies of known composition in the 
chemical laboratory, should not attempt the quantitative deter¬ 
minations relating to the blood or other animal liquids, partly 
because the operations are complicated, but principally because 
the operator has no means of detecting his mistakes.—The blood 
to be analyzed is received in four vessels, the contents of which 
are as follow :—1. Ten or twelve centimeters of blood are allowed 
to flow into a weighed porcelain capsule and covered with a 
weighed watch-glass. After weighing, the blood is evaporated 
in a water-bath, dried in the air-bath at 120° C., and the resi¬ 
due used for the determination of the total albuminous consti¬ 
tuents, fat and salts, as follows :—After standing till it is cool in 
a receiver over sulphuric acid, it is weighed. The weight, deducted 
from that of the capsule and watch-glass, gives the total solids. 
The dry residue is then pulverized in a glass or porcelain mortar 
with common alcohol (Sp. G. 890) and transferred to a small 
beaker, the mortar being subsequently carefully washed with 
alcohol, and the washings added to the quantity in the beaker. 
This done, the contents of the beaker are boiled, and the alcoholic 
solution thus obtained is poured into a small previously weighed 
filter. What remains in the beaker is similarly treated with a 
second quantity of alcohol, which is thereupon poured into the 
same filter. After carefully washing the filter with boiling 
alcohol, the filtrate together with the washings is evaporated on


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